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10th International Workshop on the Biology of Fish Gametes (IWBFG)

11-14 October, 2026, Balatonalmádi, Hungary

Preliminary program

Sunday, 11th October 2026

Registration

Welcome reception

Monday, 12th October 2026

Registration desk is open

Dr. Tamás Müller

When sperm waits in the ovary: Ovarian insemination as a novel propagation method for externally fertilising fish | Plenary lecture

Session 1 - Oocyte biology, gamete compatibility and fertilisation

Chairs: Catherine Labbé and Daniel Żarski

Uroš Ljubobratović

Does nest mating make gametes match?

Olga Bondarenko

Beyond water activation: Membrane permeability and the hidden vulnerability of fish reproduction

Coffee break

Aarón Torres Martínez

Morphological and molecular signatures of ovarian ageing in zebrafish

Buumba Hampuwo (student)

Environmentally relevant concentrations of microplastics translocate into gametes in a sex-specific manner, altering their physiology without impairing mean reproductive output in brown trout

Elvira Fatsini

Culture environment influences gonadal reproductive pathways in Mugil cephalus

Tomáš Tichopád

Why triploid zebrafish refuse to become female: Rewiring of hormonal control in sex differentiation

Yihui Mei (student)

Gamete ploidy engineering overcomes inter-family reproductive isolation in teleost fish

Lunch

Session 2 - Germline development and sex differentiation

Chairs: Uroš Ljubobratović and Oliana Carnevali

Roman Franek

Temporal redistribution of germline proliferation links developmental timing to life-history strategy in annual killifish

Xiaojuan Cao

Cdk1 deficiency triggers premeiotic endoreplication and unreduced sperm formation via the Cdk2/Ccne1–Fzr1a pathway in loach

Essaikiammal Konar

Young gonadal environment reverses male reproductive ageing

Toshiya Nishimura

Functional analysis of DND1 and NANOS3 domains during primordial germ cell-like cell induction in medaka

Coffee break

Martin Pšenička

Sturgeon embryos as a model for germline specification: Mitochondrial contributions and evolutionary insights

Taishi Shimabukuro (student)

Identification of downstream factors of DND1 and NANOS3 involved in PGC formation using TRIBE

Moe Takeuchi

Germ cells derived from haploids undergo genome doubling during the stem cell-like division phase in medaka

Juan F. Asturiano

Trials for the in vitro maturation of small-spotted Catshark oocytes

Poster Session

Tuesday, 13th October 2026

Registration desk is open

Dr. Ozren Bogdanović

Totipotency without a clean slate: epigenome dynamics across the teleost life cycle - Plenary lecture

Session 3 - Omics and molecular profiling in fish reproduction

Chairs: Rüdiger Schulz and Vanesa Robles Rodríguez

Ignacio Fernandez

REPHEAT project: Multi-omic approaches to unveil potential transgenerational effects and mechanisms of climate change in Oncorhynchus mykiss

Rafael Nobrega

Single-cell transcriptomic atlas reveals an interplay between germ cells and the immune system during the resumption of spermatogenesis in adult zebrafish

Coffee break

László Orbán

The male-to-female transformation process begins with a return to a near-undifferentiated transcriptomic state in the gonad of the protandrous Asian seabass

Emilia Arim-Galiano (student)

Thyrotropin-releasing hormone receptor as a key driver of sexual fate

Daniel Żarski

Ovulation-driven transcriptomic reorganisation defines maternal RNA provisioning in pikeperch oocytes

Joanna Nynca

Transcriptomic and proteomic signatures of early in vivo post-ovulatory ageing in pikeperch eggs

Sonali Kaththriarachchi (student)

Combined physicochemical and proteomic profiling identifies biochemical associations of oocyte quality and fertilisation outcome in Atlantic salmon

Lunch

Session 4 - Spermatogenesis and germline cell technologies

Chairs: Zoran Marinović and Martin Pšenička

Alex Kojo Datsomor

Development of an in vitro culture system for Atlantic salmon germ-cell propagation, transplantation and genetic manipulation

Marta Lombó Alonso

Short-chain fatty acids as guardians of fish spermatogenesis

Zoran Marinović

Utilisation of microfluidics for the successful enrichment of spermatogonial stem cells

Rüdiger Schulz

Photoperiod and feeding alter the timing and incidence of entering pubertal spermatogenesis in Atlantic salmon grilse

Coffee break

Sheyda Shapouri (student)

Direct evidence of the phagocytic properties of in vitro cultured zebrafish Sertoli cells toward spermatogonia

Anna Majewska

Temperature-dependent regulation of Secreted Novel AID/APOBEC-like deaminases in common carp gonads

Olivér Máté Szabó (student)

An accelerated selection method yields a pigment-deficient transgenic reporter line in zebrafish to study gonadal transformation

Poster Session

Wednesday, 14th October 2026

Registration desk is open

Dr. Rosana Estevez

Scaling up Atlantic salmon sperm cryopreservation: from research-scale trials to commercial hatchery production - Plenary lecture

Session 5 - Spermatology

Chairs: Rafael Nóbrega and Joanna Nynca

Serhii Boryshpolets

Restoring the biological meaning of CASA parameters using high-frame-rate sperm tracking

Leonor Ferrão

What defines high- and low-quality sperm samples? An integrative analysis in the European eel

Coffee break

Vitaliy Kholodnyy

Ovarian fluid enhances sperm performance but shows species-specific effects across native and introduced salmonids in Japan

Laurine Mueni

Rheological properties of fertilisation media shape sperm motility, interaction behaviours and diversification across fertilisation strategies

Zhijun Ma

Effects of short-term storage on sperm quality and epigenetic modifications in common carp

Gregory Brayan Valdivia Gutiérrez

Post-mortem sperm kinetics in jundiá: Establishing collection windows based on rigor mortis progression

Sylwia Wałdowska

Post-thaw sperm storage as a tool for identifying paternal-effect genes in Eurasian perch

Lunch

Session 6 - Aquatic germplasm conservation

Chairs: Juan F. Asturiano and Elsa Cabrita

Ákos Horváth

Is post-thaw agglutination really a problem in common carp sperm cryopreservation?

Irene Martí Gil

Connecting continents through aquatic germplasm: The AGGRC mission in a global context

Jhony Lisboa Benato

Democratising access to cryopreservation technologies: A South American perspective on open-hardware solutions

Anatolii Sotnikov

Scalable large-volume cryopreservation of fish sperm using simplified hypotonic media

Coffee break

Danilo Streit Jr

Sperm cryopreservation protocols in Serrasalmidae, the main South American native aquaculture group: A systematic review

Maria Mafalda Almeida

Robust maintenance of the paternal methylome following sperm cryopreservation and its transmission to early embryos in common carp

Closing remarks

Gala dinner

List of posters

Marina Morini

Environmental stress sensing through TRPV channels in Magallana gigas gonads under a realistic scenario: silver exposure and thermal anomalies

Eduardo Sanches

induced reproduction on nile tilapia using different hormones

Konrad Pomianowski

Melatonin and AFMK in plasma, ovarian fluid, and eggs during reproduction in farmed rainbow trout: an antioxidant role

Denise Vizziano-Cantonnet

Ancestral signals of sex: early male gene emergence in sturgeon development

Mariola Dietrich

Isolation and proteomic characterization of extracellular vesicles from carp (Cyprinus Carpio) seminal plasma

Md Sabbir Hossain

Short-term preservation techniques for zebrafish (Danio Rerio h.) sperm

Mohammad Rashed

CRISPR/Cas9-Mediated knockout of the sperm-specific CNGK channel to investigate its role in zebrafish sperm motility and fertility

Thales França

Improving european eel sperm preservation with a new extender

Thales França

Successful use of seminal plasma as extender during european sea bass sperm short and long-term storage

Luciana Ganeco-Kirschnik

Large-scale assisted fertilization for tambaqui (Colossoma macropomum) using cryopreserved semen in a breeding improvement program

Ami Aita

Post-ovulatory assembly of germ plasm reveals a distinct mechanism of germ plasm formation in medaka (Oryzias latipes)

Leonardo Alencar

Influence of broodstock origin on post-thaw sperm quality of Pogonias courbina

Yaiza Fernández Jorreto

Spermatozoa morphology in mediterranean elasmobranchs

João Ferreira

Establishment of a 3D testicular organoid system using aggrewell as a novel tool to study spermatogenesis in fish

Fernanda O'Sullivan

Spermatogenesis of an ancient fish: preliminary results from Arapaima gigas

Arpit Sivchandan

Cellular and Molecular Aspects of Testis Regeneration in Zebrafish.

Rafael Nobrega

In vivo pharmacological modulation of Notch signaling in the testes of teleost fish

Rafael Nobrega

Seeing beyond the slice: Machine learning approaches for sertoli and spermatogonial stem cell detection and quantification in 3D imaging of zebrafish testes

Yaiza Fernández Jorreto

Reproductive cycle of scyliorhinus canicula in the western mediterranean sea: What we know

Mykauana Alves Vila Nova

Preliminar transcriptomic characterization of testicular single nuclei of the Neotropical Brycon amazonicus

Catherine Labbé

Extracellular vesicles in trout seminal plasma: preparation and preliminary proteomic features

Gregory Brayan Valdivia Gutiérrez

Does sequential cryopreservation of spermatozoa in f0 and f1 compromise reproductive performance in a neotropical catfish?

Danilo Streit Jr

Post-mortem sperm cryopreservation in the endangered groovebelly stingray (Dasyatis hypostigma)

Jhony Lisboa Benato

Sperm cryopreservation does not alter larval or juvenile behavior in Rhamdia quelen

Elsa Cabrita

The use of extracellular vesicles (EVS) in fish sperm cryopreservation: The gilthead seabream model

Joanna Nynca

A tissue-resolved transcriptomic atlas of the reproductive axis in pikeperch (Sander Lucioperca)

Overview